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bibi

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Posted 15 June 2005 - 07:10 PM

:crybaby: Help! I am stack.
How to put the right concentration of chlorine for desinfection of salads.
Which instruments are used?
How long?
How can I make sure my salad is free or reduced level of bacterias


Simon

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Posted 16 June 2005 - 07:08 PM

Now you've got me stuck Bibi. :helpplease: Witch the resident micro expert at the forums stops by pretty regular and hopefully will be able to answer your questions.

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Simon


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Witch

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Posted 20 June 2005 - 08:20 AM

:crybaby: Help! I am stack.
How to put the right concentration of chlorine for desinfection of salads.
Which instruments are used?
How long?
How can I make sure my salad is free or reduced level of bacterias

<{POST_SNAPBACK}>

Hallo all,
thank you Simon for the roses... :oops:
aqueous solutions of sodium hypochlorite or hypochlorous acid are typically used to sanitize fresh fruits and vegetables.
For chlorinated water I found 200 microg/ml...in the literature.
Another method seems to be the following:
Chlorine dioxide (ClO2) gas generated by a dry chemical sachet was tested against foodborne pathogens on lettuce leaves. Lettuce leaves were inoculated with cocktail of three strains each of Escherichia coli O157:H7, Listeria monocytogenes, and Salmonella typhimurium and treated with CLO2 gas for different times. Total ClO2, generated by the gas packs was 4.3, 6.7, and 8.7 mg after 30 min, 1 h, and 3 h of treatment, respectively. Inoculated lettuce leaves exposed to ClO2 gas for 30 min experienced a 3.4-log reduction in E. coli, a 4.3-log reduction in Salmonella Typhimurium, and a 5.0-log reduction in L. monocytogenes when compared with the control. The ClO2, gas sachet was effective at killing pathogens on lettuce without deteriorating visual quality. Therefore, this product can be used during storage and transport of lettuce to improve its microbial safety.
A milder method is to use ozonated water: :thumbup:
ozonated water containing 5 ppm of ozone reduced viable aerobes in lettuce 1.5 log CFU/g within 10 min.
Wherefrom you can get the instruments :dunno:
Testing is done via common microbiological methods and counting cells...
If you need more help, just send me a message!
:bye:


Simon

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Posted 20 June 2005 - 07:47 PM

Thanks Witch, I think you've just about got it right. ;)

Here have another bunch of flowers. :potplant:

Sorry it's the best I could do.

Regards,
Simon


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Charles Chew

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Posted 21 June 2005 - 06:27 AM

Inadequate control on ozonation of water may result in bromide residual which is carcinogenic and this may cross contaminate the vegetables that are immersed.

There has been a lot of research in this area. IMO, a very interesting subject.

Charles Chew


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bibi

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Posted 27 June 2005 - 08:16 PM

:clap:

Inadequate control on ozonation of water may result in bromide residual which is carcinogenic and this may cross contaminate the vegetables that are immersed.

There has been a lot of research in this area. IMO, a very interesting subject.

Charles Chew

<{POST_SNAPBACK}>


thank you guys I am still searching as well. :clap:
I found a paper PH meter to dip in chlorinated water to indicate a range of purpule colour to indicate 100-200ppm which should a safe level.
What about free chlorine? :dunno:


Charles Chew

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Posted 28 June 2005 - 03:21 AM

Your test should be based on free available chlorine. The supplier of your industrial grade hypochlorite should provide you with these information. Be careful with the dynamic degradation of chlorine as they are a very sensitive agent. On application, read the MSDS and dilution instruction carefully.

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Charles Chew


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bibi

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Posted 04 July 2005 - 02:59 PM

Hi

I think I will get all the informations from CCFRA Guideline No 38 (2002)
The use of chlorine in fresh produce washing.
:clap: thanks



Simon

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Posted 04 July 2005 - 08:41 PM

It would be useful if you could let us know the critical points from the guideline Bibi.

Regards,
Simon


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