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Testing for Vibrio Cholerae

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#1 kieu nguyen

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Posted 09 June 2016 - 04:03 AM

Please help me. When I confirm presumptive vibrio cholerae on TCBS agar (have yellow colour) by test salt tolerance and biochemical test. While biochemical tests correct for virbrio cholerae but salt tolerance is wrong, 0% NaCl tube don't have turbidity. So can I conclude that presumptive colony is vibrio cholerae? Thanks so much


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#2 Charles.C

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Posted 09 June 2016 - 05:01 AM

Please help me.

when I confirm presumtive vibrio cholerae on TCBS agar (have yellow colour) by test salt tolerance and biochemical test. while biochemical tests correct for virbrio cholerae but salt tolerance is wrong, 0% NaCl tube don't have turbidity. so can I conlude that presumtive colony is vibrio cholerae?. thanks so much

 

No.

 

For example -Table 2 -

http://www.fda.gov/F...s/ucm070830.htm


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Charles.C


#3 kieu nguyen

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Posted 09 June 2016 - 05:49 AM

Thanks you so much for your reply.

But according to table 2 in your links site, the percentage of Vibrio cholerae have growth in tryptone 1% (no add NaCl) is 99,1 %, not 100%. so whether bacteria in my case is belong to 0,9 % that don't growth in media no salt.


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#4 Charles.C

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Posted 09 June 2016 - 06:33 AM

Thanks you so much for your reply.

But according to table 2 in your links site, the percentage of Vibrio cholerae have growth in tryptone 1% (no add NaCl) is 99,1 %, not 100%. so whether bacteria in my case is belong to 0,9 % that don't growth in media no salt.

 

I guess it's yr choice as to whether to agree with FDA or not.

 

Might suggest you also investigate a serological test on a pure culture.

IMEX biochemical is usually used as a screening procedure unless confirmatory serological not available/reliable.

Personally i have used growth on CLED as an equivalent (no salt) screening step for VC along with tests similar to BAM.

 

VCh non-cholera vibrios also exist of course.

 

There are a multitude of biochemically similar vibrio species. V.para can be even more problematic regarding identification/pathogenicity.


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Charles.C


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