Jump to content

  • Quick Navigation
Photo
- - - - -

How I possibly report the result of this PDA plate?


  • You cannot start a new topic
  • Please log in to reply
10 replies to this topic

#1 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 05 November 2017 - 11:30 AM

Could someone please help me on this Yeast and Mold count trick?
Suggest me some sources where I can find some information regarding the Mold count..bf6b8bbc70570a08b0a1bbf7b58ead00.jpg

Sent from my GT-N7100 using Tapatalk


  • 0

#2 Packaging QA

Packaging QA

    Grade - Active

  • IFSQN Active
  • 7 posts
  • 1 thanks
1
Neutral

  • New Zealand
    New Zealand

Posted 06 November 2017 - 01:00 AM

Report as 'SPR' = spreader. Repeat the test , check plate at 48h , 72h and put a spot on the centre of juvenile colonies (dont open lid of plate). To get like that would usually take 5 plus days , but colonies would be smaller , visible and able to be counted much earlier.


  • 0

Thanked by 1 Member:

#3 dfreund

dfreund

    Grade - AIFSQN

  • IFSQN Associate
  • 32 posts
  • 7 thanks
1
Neutral

  • United States
    United States

Posted 08 November 2017 - 04:25 PM

If this is obviously outside of tolerance limits, I would mark it TNTC (Too Numerous To Count) and mark it a sample that failed.


  • 0

#4 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 10 November 2017 - 09:07 PM

If this is obviously outside of tolerance limits, I would mark it TNTC (Too Numerous To Count) and mark it a sample that failed.

Thank you for the reply, dfreund..
This is spices mix sample's 10^3 dilution plate. All the lower dilutions are like this. Would you suggest to plate out higher dilutions so that would enable to get some colony distinctly?
Is there any supporting document regarding a detailed how-to-do method for Yeast and Mold count. I searched many standards but couldn't find one..

Sent from my GT-N7100 using Tapatalk
  • 0

#5 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 10 November 2017 - 09:15 PM

Report as 'SPR' = spreader. Repeat the test , check plate at 48h , 72h and put a spot on the centre of juvenile colonies (dont open lid of plate). To get like that would usually take 5 plus days , but colonies would be smaller , visible and able to be counted much earlier.

Thank you "Packaging QA" (that's a crazy name!!!)

This is a 10^3 dilution 72 hour plate of a spices mix product. Why it is like this in your opinion? Is it because the species is fast growing or is denote the higher number present? Would a higher dilution help?
It seems to me as A.niger growth. I expect your expert opinion on this..
Thanks in advance,

Sent from my GT-N7100 using Tapatalk
  • 0

#6 dfreund

dfreund

    Grade - AIFSQN

  • IFSQN Associate
  • 32 posts
  • 7 thanks
1
Neutral

  • United States
    United States

Posted 10 November 2017 - 09:16 PM

We are not doing this in house but I expect this is good guidance:

 

https://www.fda.gov/...s/ucm071435.htm


  • 0

#7 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 10 November 2017 - 09:25 PM

We are not doing this in house but I expect this is good guidance:

https://www.fda.gov/...s/ucm071435.htm

Thank you dfreund, for the input.
I had seen this FDA manual but the detail about my confusion is specifically not answered there. The overall growth on the surface. Would you say it might be due to the higher number present in the sample, possibly? Or this might be the nature of the Mold?


Sent from my GT-N7100 using Tapatalk
  • 0

#8 Charles.C

Charles.C

    Grade - FIFSQN

  • IFSQN Moderator
  • 12,592 posts
  • 3281 thanks
350
Excellent

  • Earth
    Earth
  • Gender:Male
  • Interests:SF
    TV
    Movies

Posted 12 November 2017 - 09:49 AM

Hi Navas,

 

Y&M not my direct experience but various approaches to yr OP appear possible, eg -

 

(1) Use Petrifilm for which counting advice is supplied (ymc1,2 below) -

(2) inc dilution, eg ymc3

 

For some theory on handling "upper limits" can try ymc4.

 

Attached File  ymc1 Petrifilm Y&M Guide(1).pdf   324.57KB   2 downloads

Attached File  ymc2 - Petrifilm Y&M Guide(2).pdf   319.52KB   1 downloads

Attached File  ymc3 - Micro.examination -total colony number (Petrifilm).pdf   159.16KB   2 downloads

Attached File  ymc4 - accuracy plate counts.pdf   3MB   2 downloads

 

 

 

 

 

 

 

 


  • 0

Kind Regards,

 

Charles.C


#9 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 12 November 2017 - 04:09 PM

Hi Navas,

Y&M not my direct experience but various approaches to yr OP appear possible, eg -

(1) Use Petrifilm for which counting advice is supplied (ymc1,2 below) -
(2) inc dilution, eg ymc3

For some theory on handling "upper limits" can try ymc4.

attachicon.gifymc1 Petrifilm Y&M Guide(1).pdf
attachicon.gifymc2 - Petrifilm Y&M Guide(2).pdf
attachicon.gifymc3 - Micro.examination -total colony number (Petrifilm).pdf
attachicon.gifymc4 - accuracy plate counts.pdf

I really appreciates your help, Charles.
Now I am getting more focused to what answer I search for. I think, these documents would help to convince my Superior. I had that Petrifilm one, but others also feels really helpful..
Thank you again..

Sent from my GT-N7100 using Tapatalk
  • 0

#10 Packaging QA

Packaging QA

    Grade - Active

  • IFSQN Active
  • 7 posts
  • 1 thanks
1
Neutral

  • New Zealand
    New Zealand

Posted 12 November 2017 - 06:55 PM

Thank you for the reply, dfreund..
This is spices mix sample's 10^3 dilution plate. All the lower dilutions are like this. Would you suggest to plate out higher dilutions so that would enable to get some colony distinctly?
Is there any supporting document regarding a detailed how-to-do method for Yeast and Mold count. I searched many standards but couldn't find one..

Sent from my GT-N7100 using Tapatalk

You have a mold that is aggressively growing on the surface of the plate. The fact your lower dilutions look like this also doesnt necessarily mean there are high numbers. As I said, ask that the lab to count at 48/72H to spot the juvenile colonies before they get to the point where they are mature and spread over one another.


  • 0

#11 navas

navas

    Grade - Active

  • IFSQN Active
  • 15 posts
  • 0 thanks
0
Neutral

  • India
    India

Posted 13 November 2017 - 05:44 AM

You have a mold that is aggressively growing on the surface of the plate. The fact your lower dilutions look like this also doesnt necessarily mean there are high numbers. As I said, ask that the lab to count at 48/72H to spot the juvenile colonies before they get to the point where they are mature and spread over one another.

Yes, I got the point now..

Thank you my friend....

Regards

-Navas


Sent from my GT-N7100 using Tapatalk
  • 0




0 user(s) are reading this topic

0 members, 0 guests, 0 anonymous users