Posted 08 June 2009 - 06:21 AM
A bit of background ATP stands for Adenosine-5-triphosphate, it is the fundamental molecule of energy in a cell. It releases energy by breaking down to ADP(Adenosine diphosphate) and AMP(Adenosine monophosphate). ATP can be found in bacteria and food debris.
ATP Bioluminescence works by light being generated by a reaction of oxidising Luciferin(a light emitting biological pigment) with ATP and Oxygen catalysed by the enzyme Luciferase.
The technology uses Luciferin and Luciferase from the Firefly because it is known that they require ATP to be present in order for light to be produced.
The instruments measure the amount of light emitted which should be proportional to the amount of ATP present from the swab/sample.
As ATP is present in Food Debris and Bacteria ATP swabbing is an excellent indicator of hygiene and is used in many factories to positively release filling machines as clean. Setting up of a swabbing system requires doing some background work to establish readings for clean equipment and then setting limits. I first used this sytem to release filling machines 15 years ago and improved our micro results 100 fold. I am a big fan as it enables you to control the process rather than waiting for a couple of days to see if the micro swab results were okay.
I have also worked on ATP technology to test product and have used it to reduce the release of UHT products from 7 days to 3 days. If anyone wants any more details then let me know.
Regards,
Tony