How do you get swabs from the production area using sponges for the Listeria and Salmonella studies? I mean presence and number.
For example, if you hydrate the sponge in 10 ml of neutralizing liquid, taka a 100 cm2 swab and pour in 90 ml fraser broth (Listeria) or add 25 ml of neutralizing liquid and pour in 225 ml Peptone Water (Salmonella). This is dilution 101. And how to make a dilution of 100.
I found a message that the smear surface is equal to the amount of diluent. In another source, for 100 cm2 sponge swab, add 20 ml of diluent.
Can someone explain it to me?