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What is mean by the coliforms negative in 0.1gram?

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myocrban

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Posted 24 April 2014 - 03:00 AM

Dear all:

 

 

           Our products export to Russian and the coliforms limit is negative in 0.1gram, I want to know what test method to be used?

 

           Does anyone help me?

 

 

           thank you!



Charles.C

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Posted 24 April 2014 - 03:25 AM

Dear all:

 

 

           Our products export to Russian and the coliforms limit is negative in 0.1gram, I want to know what test method to be used?

 

           Does anyone help me?

 

 

           thank you!

Usually means "Not detected" in sample size of 0.1g

 

ie <1cfu / 0.1g

ie, equivalently,  <10cfu/1g or similarly for other sample sizes

But Russian buyers might have a different mathematical theory. :smile:

 

Normally at this level, it is recommended to use a MPN method such as detailed in USFDA-BAM (on-line available) so as to improve accuracy compared to plate count technique.

 

But Russia may have its own local standard procedure.

 

Rgds / Charles.C


Kind Regards,

 

Charles.C


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Zeeshan

myocrban

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Posted 25 April 2014 - 09:42 AM

Usually means "Not detected" in sample size of 0.1g

 

ie <1cfu / 0.1g

ie, equivalently,  <10cfu/1g or similarly for other sample sizes

But Russian buyers might have a different mathematical theory. :smile:

 

Normally at this level, it is recommended to use a MPN method such as detailed in USFDA-BAM (on-line available) so as to improve accuracy compared to plate count technique.

 

But Russia may have its own local standard procedure.

 

Rgds / Charles.C

thanks Rgds / Charles.C



AS NUR

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Posted 23 May 2014 - 12:57 AM

yes i agree with charles.. and the other method to count that is Total Plate count.

Rgds

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mapry2

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Posted 23 May 2014 - 02:33 AM

Also you can interpret not detected in 1/10 dilution



Charles.C

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Posted 23 May 2014 - 02:46 AM

Also you can interpret not detected in 1/10 dilution

 

Assuming that the quantity of analysed portion of yr dilution contains 0.1g of sample, yes.

 

Strictly speaking, one should be relating to a sampling plan of course. And preferably a MPN-type analytical procedure.

 

Rgds / Charles.C


Kind Regards,

 

Charles.C




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