Membrane Filtration Technique

Hi all,

does anyone know how to prepare the sample incoming raw material such as fructose and sugar for membrane filtration technique?

How much the sample we need to dilute it before we filter?



TQ.

Hi all,

does anyone know how to prepare the sample incoming raw material such as fructose and sugar for membrane filtration technique?

How much the sample we need to dilute it before we filter?



TQ.

Dear Ati..

is that for dirt test or micro test ?

Dear As Nur,

it's for micro test.

Dear As Nur,

it's for micro test.

International methods for sugar analysis (including microbiological methods) are published by ICUMSA (International Commission for Uniform Methods in Sugar Analysis, see http://www.icumsa.org/ ).


The basics of the method (assuming a filterable sample) for aerobic plate counts for bacteria, yeasts or moulds are:


- 10g dry sugar equivalent dissolved in 100ml total volume of sterile demineralised water

- filter through 0.45 micron pore size sterile membrane

- place filter onto suitable media and incubate aerobically

Dry sugar equivalent is aimed at testing the same amount of sucrose in diverse products such as dry sugar and liquid sugar (which is 2/3rds sugar & 1/3rd water, so 10g dry sugar equivalent is 15g liquid sugar).


Hope that helps,
Marco (sugar company microbiologist)

Dear Marco,

its really me a lot. thanks