How to prepare 60 microM of DPPH solution?

Hello everyone.

Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?

Thank you

Hello everyone.

 

Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?

 

Thank you

Hi rossy,

I assume you are familiar with diluting techniques.

I assume you are not requiring ultimate accuracies, otherwise maybe read this link –

http://www.pharmtech...-your-dilutions

http://chemistry.sta...olutions-method

M = molar

mM = millimolar

mcM = micromolar

Afaik, the MW of DPPH is 394.33g

A 1 molar solution is a solution in which 1 mole of a compound is dissolved in a total volume of 1 litre.

A 1 molar solution DPPH will require 394.33g in 1 litre of solution

Approximately –

Weigh 0.394g of DPPH, dissolve  and make up to 1000ml with solvent = 1mMsolution DPPH = "A"

Pipette 1ml of A and make up to 1000ml with solvent = 1mcM solution = "B"

60ml of B will contain 60micromoles of DPPH