Enterobacteriaceae to validate sanitation program
Hello every one,
I am trying to validate the sanitation program in our Rice and Grain plant. and we decide to test Enterobacteriaceae as an indicator. when we receive the results from the lab that used the method AOAC 2003.1 (Petrifilm) it was about 100,000/swab. my question is what is the real unite as i have to compare it to UCF/cm^2 and what is the limit for non RTE product (I found only limit for RTE product)
Thank you
Hello every one,
I am trying to validate the sanitation program in our Rice and Grain plant. and we decide to test Enterobacteriaceae as an indicator. when we receive the results from the lab that used the method AOAC 2003.1 (Petrifilm) it was about 100,000/swab. my question is what is the real unite as i have to compare it to UCF/cm^2 and what is the limit for non RTE product (I found only limit for RTE product)
Thank you
Hi houdaR,
I presume UFC = cfu.
I presume 100,000/swab = 100,000 cfu/swab
A little info is required -
(1) What "item" was swabbed ?
(2) What was the area swabbed ?
(3) If the "item" was a food contact surface (fcs), was the area to be swabbed cleaned/sanitised before testing ?
If the item was a fcs published micro. limits are often based on a just cleaned/sanitised surface . For some data see this Post/thread -
http://www.ifsqn.com...ces/#entry60958
The value for Enterobacteriaceae in cfu/cm2 for a fcs as determined above would normally be low.
Thank you Charles C.
The swabs was done for Food contact surfaces after cleaning. The link you refer to is for RTE products. and all the limits are given CFU per cm2 not per swab.
Thanks
Thank you Charles C.
The swabs was done for Food contact surfaces after cleaning. The link you refer to is for RTE products. and all the limits are given CFU per cm2 not per swab.
Thanks
Actually link not RTE products when i test it. It is for FCS.?
To calculate cfu per cm2 you need the Area as per No.2 If you don't know it, you will need to ask the lab. (it's often 10cm x 10cm but it may depend on precisely what was swabbed, hence query No.1)
Thank you Charles
Your explanation is very helpful :smile: