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Enterobacteriaceae temperature

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#1 lilii

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Posted 23 September 2020 - 06:00 PM

Hi,
 
What is the difference in using the temperature of 30 degrees or 37 ?
30 degrees is for technological reasons, 37 for hygienic reasons in the case of Eneterobacteriaceae. 
Does it mean that we use temperature 37 for swabs and 30/37 for food?
Coliforms  30 degrees technological reasons, 37 health considerations.
 
 
Best Regards,
 
 
 


#2 Charles.C

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Posted 23 September 2020 - 06:14 PM

 

Hi,
 
What is the difference in using the temperature of 30 degrees or 37 ?
30 degrees is for technological reasons, 37 for hygienic reasons in the case of Eneterobacteriaceae. 
Does it mean that we use temperature 37 for swabs and 30/37 for food?
Coliforms  30 degrees technological reasons, 37 health considerations.
 
 
Best Regards,

 

The choice of temperature relates to the likelihood of growth by psychrophilic and mesophilic bacteria respectively.


Kind Regards,

 

Charles.C


#3 olenazh

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Posted 23 September 2020 - 06:15 PM

Not clear to me: what temperatures are you talking about? Killing temp? Than, it's minimum 70 C. And what temperatures for swabs and food? What products do you manufacture? Please, clarify.



#4 Charles.C

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Posted 23 September 2020 - 06:38 PM

Not clear to me: what temperatures are you talking about? Killing temp? Than, it's minimum 70 C. And what temperatures for swabs and food? What products do you manufacture? Please, clarify.

Probably Incubation temperatures.


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Charles.C


#5 olenazh

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Posted 23 September 2020 - 06:40 PM

Right, I've checked - sorry for misunderstanding:)



#6 EagleEye

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Posted 23 September 2020 - 07:25 PM

Selecting right incubation temperature, as you mentioned- would help certain set of organisms to grow prominently over the others(to some extent).

In this case, 37 is the right temperature to choose for suspected human pathogen coliforms over the others of the same group(health concerns). This however just a preliminary information and needs a series of other tests too for the confirmation.

30 would allow a range of other coliforms (down towards the other end of mesophilic range) also to grow which would be giving to you somewhat more coliform count(if any present). These all are not necessarily be pathogenic and useful to evaluate a hygienic situation generally.

There are no rigid lines of separation between all these but can assume closeness to accuracy and add to assumptions based on other factors, case by case.


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#7 Charles.C

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Posted 23 September 2020 - 07:54 PM

Selecting right incubation temperature, as you mentioned- would help certain set of organisms to grow prominently over the others(to some extent).

In this case, 37 is the right temperature to choose for suspected human pathogen coliforms over the others of the same group(health concerns). This however just a preliminary information and needs a series of other tests too for the confirmation.

30 would allow a range of other coliforms (down towards the other end of mesophilic range) also to grow which would be giving to you somewhat more coliform count(if any present). These all are not necessarily be pathogenic and useful to evaluate a hygienic situation generally.

There are no rigid lines of separation between all these but can assume closeness to accuracy and add to assumptions based on other factors, case by case.


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"Coliform" is not regarded as a pathogen (benefit of the doubt). Hence its problematicity as far as making predictions go. Similarly Enterobacteriaceae.

 

Coliforms has become somewhat obsolete as a hygiene indicator although some Locations/Industries won't let go.

 

IIRC, 30degC is particularly advantageous to psychrophilics. as far as APC is concerned. The choice here may be more oriented to encouraging growth of  species related to spoilage mechanisms such as in seafood.

 

I cannot recall ever seeing 30degC used for coliform measurements ? Or Enterobacteraceae ? (maybe in Slovenia ?)


Kind Regards,

 

Charles.C


#8 EagleEye

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Posted 23 September 2020 - 08:40 PM

It is pathogenic Eneterobacteriaceae, I wanted to say.
(Coliforms' benefit of doubt is not disregarded, though)

In the case of Eneterobacteriaceae, I do not find any other purpose in using this two different temperature other than I mentioned above ( also still not aware about the intention of this post).

As you're rightly said, many of those people are so concerned with the coliform count like anything.

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