Hi ss2010,
In my personal opinion, the swab test on the washed hands is not called risk assessment. It seems just a kind of validation/verification for the temperature of water you selected.
In ISO 9000 the concept "validation" & "verification" are addressed and defined. Validation is confirming if the method is able to achieve the expecting result;
And verification is verifying whether the real operation is performed against your established control measures and achieve the specification.
The risk assessment is a kind of statistic method to illustrate the risk of the hazard to be controlled by factories. Codex hazard analysis(
HACCP) is a kind of risk assessment. After done this, you could establish
CCPs for those "significant" hazards(with high risk scores). Normally in my country, swab test is conducted in the processing room, but not directly after hand washing. It is one verification measure to the established SSOP for hand cleaness.
Back to your issue, my personal opinion, if you want to validate/verify effectiveness of the tap water temperature on the washed hands, TPC and fecal indicator coliforms can be tested. Other additional pathogen tests based on the kind of products. e.g. Salmonalla etc.
Finally, I think the sole validation to the water temperature is not wisable. Because, hand sanitizing normally divided into five steps: wash-liquid soap cleaning-wash-disinfectant-wash. Combination of such five steps result in the final loading of microbees(of course original culture loading is another factor for final loading). Yes, the higher water temperature, the easier removing the bactera from hands. But I propose to consider this factor combining with other factors. Then you can find your what you want.
Only my five cents opinion,
Best regards
Jason