Hello everyone,
this time I need your help on autoclave settings. we used autoclave to sterilize the media, we set cycle as 121 degrees Celsius for 15 minutes, when our cycle completed we observed that our media was completely burnt (as its color supposed to be light yellow but it was blackish brown), autoclave tape changed its color to slightly darker shade but it was not fully black, the technician says we need to set the off set temperature in autoclave which has some relationship with room temperature or lab temperature, but I could not understand his point. if you guys can help me on this.
thanks