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Determination of polyphenol in olive oil?

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#1 hazemhandsa


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Posted 25 June 2009 - 11:03 AM

plz i am want any research for determination of polyphenol in olive oil and olive pomace
and i want to know how i can prevent the enzymatic reaction in quarters artichoke through the production operations.
thank u for ur cooperation

Eng - Hazem

#2 SaRaRa


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Posted 25 June 2009 - 11:36 PM

Hello Hazem,

I found the following with a quick search:

Polyphenols determination in olive oil samples based on a thick film voltametric sensor and a tyrosinase biosensor

Handbook of Olive Oil

Olive Oil - Minor Constituents and Health

I will look more on my notebooks when I 'll have more free time. I am sure I have more detailed info on this somewhere!

#3 SaRaRa


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Posted 26 June 2009 - 01:49 PM

The information below are a very reliable source because I translated them from my university notebooks of Fats and Oils Technology class which were edited and tought by Dr. Apostolos Kyritsakis (Msc., Ph.D. in University of Michigan) who is a well known and respected researcher and professor in Greece and worldwide. He has edited many awsum books about oils and especially about olive oil. I strongly suggest that if you are interested you should try and find them because they are truly amazing!

Determination of the phenolic substances in olive oil

A. Generally about phenols

Phenols are natural antioxidant substances that can be found in certain plant oils and mainly in olive oil. Phenols are water soluble substances and thus during the processing of the olives, a big portion migrates to the water phase. A quantity though remains in the oil and it affects its stability in oxidation. The above show that its really important to know the phenolic content of an oil.

The determination of phenols with the method described here, is based on the formation of colour using the Folin-Ciocalteau reactant agent which in the presence of sodium carbonate solution, reduces the phenols.

B. Reactant agents and solutions used

1) Methanol solution in water (60%)
2) Folin-Ciocalteau (2 N)
3) Sodium carbonate solution (35%)
4) Hexane

C. Procedure

1) In an 250 ml Erlenmeyer vial/bottle we measure 10 g of oil and we add 50 ml of hexane.

2) We add 20 ml of the methanol solution and we stir the mixture thoroughly for 2 minutes in a shaker. The process is repeated for 2 more times. Every time, after the separation of the phases, the methanolic phase is being removed and collected in a glass.

3) After the 3 extraction, the methanolic extracts are evaporized wutg a rotary evaporator at 70 C until there is only dry residue left.

4) The dry residue is dissolbed in 1 ml of methanol.

D. Calculation of the phenol content

1) 0,1 ml of the methanolic extract is added into a 10 ml round vial.

2) 5 ml of distilled water and 0,25 ml of Folin-Ciocalteau are added and the mixture is stirred thoroughly.

3) 3 min later we add 1 ml of sodium carbonate solution and we fill with distilled water.

4) After 1 hour we measure the absorbance of the blue colour that will form (if there are any phenols that is), at 725 nm. As a control is used Folin-Ciocalteau solution.

For the quantitative determination of phenols that are present in the sample, a standard curve is constructed using pure caffeic acid solutions with concentrations ranging from 0-100 μg / 10 ml of solution.

NOTE: The final content of the phenols is usually given in ppm. The result should be expressed towards the phenolic acid that is being used fot the construction of the standard curve. Except caffeic acid, olive oil contains other phenolic acids such as cumaric acid and protocatechuic acid.

#4 Charles.C


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Posted 26 June 2009 - 01:55 PM

Dear Sarara,

Marvellous stuff :clap: :clap:

Now I remember why I hated Organic Chemistry. :biggrin:

Thks all yr efforts.

Rgds / Charles.C

Kind Regards,



#5 SaRaRa


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Posted 26 June 2009 - 01:59 PM

Now about artichokes I just found the following links. Maybe you can find something interesting there.

Rapid hydrolization of Jerusalem Artichoke

Postharvest Technology of Horticultural Crops

Biology and Chemistry of Jerusalem Artichoke

Jerusalem Artichoke

Breeding of Seed-planted Artichoke


P.S. Thanx Charles! Tis always a pleasure to help whenever I can! :biggrin:

Edited by SaRaRa, 26 June 2009 - 02:00 PM.

#6 Simon


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Posted 02 July 2009 - 06:41 PM

Hazem was the research and information provided by SaRaRa of any use? Some feedback would be nice.


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