Dear Tony / GMO,
I wouldn't want to take the risk but I suspect that any E.coli would be killed if frozen. Maybe I would validate my theory.
Based on comments for food matrices and general microbiological obsrvations, a probability factor should perhaps be included but this statement seems rather optimistic to me. Have you seen any accessible validations ?
Eg for food
Bacteria can grow in the danger zone between 4°C and 60°C (40ºF to 140°F). Keep cold food cold at or below 4°C (40°F).
Refrigeration at or below 4°C (40°F) slows down most bacterial growth. Freezing at or below -18°C (0°F) can stop it completely. (But remember: refrigeration and freezing won't kill bacteria. Only proper cooking will do that!)
i second the idea that freezing does not kill beasties. i also work in a lab, and not only do we freeze E. coli at -80 degrees celsius (-112 F) for later use, we also freeze yeast. one kind of yeast, S. cerevisiae, is what is used in bread and beer. you definitely encounter it, but it is non-pathogenic. another kind, Candida albicans, is the variety that causes yeast infections. it is part of a woman's usual flora, but shifting the balance down there leads to bad things. wash your clothes!
posted by al24lola on February 23rd 2010 at 4:59pm
al24lola: note, though, that when you freeze lab coli or yeast you have to resuspend it in a ~25% v/v or greater glycerol solution, otherwise you get pretty terrible viability (i.e., a lot of them die... which means that freezing should actually have the desired effect here).
http://www.re-nest.c...me-hacks-109266 (!! )
Rgds / Charles.C