Hi everybody!!
Right now I'm trying to determine the content of methanol in juice drinks by gas chromatography. More concretely I'm studying how degrades the Velcorin (DMDC) in juices, and how this antimicrobial produces methanol by hydrolisis in the juices.
So, for studying how is increasing the content of methanol, I need to determine the concentration in the drink every 40 min, so every 40 min I inject a sample to the chromatograph.
The problem is the concentration of sugars in the drink, which obturates the column of the chromatograph and makes dirty the glass liner. So, due to this, when I start the experiment I get a lot of bad determinations, with no sense (lower concentrations, no peaks or a lot of interferences in the chromatograms...).
I have tried to solve this cutting the column every day I start an experiment, but it's not enough, I can't get solid results and see how is increasing the content of methanol.
I have tried also to make it by Static Headspace, but I haven't got good results too: bad chromatograms with no peaks and no sensibility by the method.
Before inject the sample I centrifugue it (11000 rpm during 5 min), and then pressed through a 0,45 um porus.
I have thought in make it by Dynamic Headspace, because I have read that is a better way to analyse dirty samples.
I don't know what else can I try...
I'm a little bit desperate... If you could give me some advice, it would be great!!!
Thanks in advance to everybody!!!!
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