Jump to content

  • Quick Navigation
Photo

How to determine sample size to be confident of result?

Allergen Sampling

  • You cannot start a new topic
  • Please log in to reply
6 replies to this topic

#1 Konesh Arunasalam

Konesh Arunasalam

    Grade - Active

  • IFSQN Associate
  • 14 posts
  • 0 thanks
0
Neutral

  • Canada
    Canada

Posted 03 January 2014 - 04:01 PM

Hi,

I like to know how to sample finished product or ingredient so the sample to be tested is representative of the product as a whole so it will increase confidence in subsequent test result. This is need for allergen validation of finished products and to verify incoming ingredients are gluten-free.

Thanks

Konesh



#2 Rokobia

Rokobia

    Grade - Active

  • IFSQN Active
  • 1 posts
  • 0 thanks
0
Neutral

  • Canada
    Canada

Posted 03 January 2014 - 05:15 PM

A composite sample of all product is a representative of the products. Advice is to take samples from start, middle and end of production.

A positive will inform detailed testing.

 

Thanks

 

Pinks. 



#3 George @ Safefood 360°

George @ Safefood 360°

    Grade - SIFSQN

  • Corporate Sponsor
  • 374 posts
  • 317 thanks
23
Excellent

  • United States
    United States
  • Gender:Male
  • Location:Ireland and USA

Posted 04 January 2014 - 04:12 PM

Take a look at the BSi 6001 for the standards developing sampling plans.

 

George



#4 Sigrid B

Sigrid B

    Grade - Active

  • IFSQN Active
  • 13 posts
  • 5 thanks
0
Neutral

  • Earth
    Earth
  • Gender:Female

Posted 06 January 2014 - 02:03 AM

A composite sample of all product is a representative of the products. Advice is to take samples from start, middle and end of production.

A positive will inform detailed testing.

 

Thanks

 

Pinks. 

We also did composite sampling. You can also ask your allergen test kit supplier if they have different recommendations based on your facilities or the nature of your product.

 

Regards,

Sigrid



#5 Konesh Arunasalam

Konesh Arunasalam

    Grade - Active

  • IFSQN Associate
  • 14 posts
  • 0 thanks
0
Neutral

  • Canada
    Canada

Posted 06 January 2014 - 06:57 PM

Thanks everyone for your valuable imput.

Konesh



#6 Charles.C

Charles.C

    Grade - FIFSQN

  • IFSQN Moderator
  • 17,349 posts
  • 4833 thanks
943
Excellent

  • Earth
    Earth
  • Gender:Male
  • Interests:SF
    TV
    Movies

Posted 07 January 2014 - 05:25 AM

Dear konesh,

 

Yr question as far as "representative" is involved is primarily statistical. Detection is a different aspect

 

And statistically similar to sampling for Salmonella if you are referring to an isolated lot of individual packages of product derived from  (possibly) various sources.

 

The typical statistical requirement is one of determining  a sample size such that one can state that there is, for example, a 95% probability (ie confidence level)  that the level of contamination in the lot is less than X%. Numerous publications detail the solution for this, eg the classic publication Microorganisms in Food Vol.1(?)  from ICMSF (later updated in vol8 (?) which latter may be available on-line as google book).

 

In practice, some interpretations arbitrarily set a standard such that compliance (eg null detection result for 15 random samples) allows one to "claim" that the lot is "free" of Salmonella. Strictly speaking, this is of limited statistical meaning of course. :smile:

 

Related extracts from some publications may also exist on this forum if you do a little searching.

 

Rgds / Charles.C


Kind Regards,

 

Charles.C


#7 moskito

moskito

    Grade - SIFSQN

  • IFSQN Senior
  • 361 posts
  • 72 thanks
14
Good

  • Germany
    Germany
  • Gender:Male

Posted 11 January 2014 - 12:30 PM

Hi,

 

I agree with Charles C., salmonella sampling plans might be helpfull (see BAM or ICMSF). But here you have to keep in mind that the statistic behind that is, that you test on "absent" i.e. you can pool samples because you are looking for a self propagating (living) system. Dilution below LOD is no a question in that case (with some limits), but with allergens as analyte that is possible if sample is not homogeneous contaminated.

 

Rgds

moskito






0 user(s) are reading this topic

0 members, 0 guests, 0 anonymous users

EV SSL Certificate