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How to prepare 60 microM of DPPH solution?


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#1 rossy syida

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Posted 05 June 2016 - 04:16 AM

Hello everyone.

 

Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?

 

Thank you



#2 Charles.C

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Posted 05 June 2016 - 07:17 AM

Hello everyone.

 

Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?

 

Thank you

 

Hi rossy,

 

I assume you are familiar with diluting techniques.

I assume you are not requiring ultimate accuracies, otherwise maybe read this link –

http://www.pharmtech...-your-dilutions

http://chemistry.sta...olutions-method

 

M = molar

mM = millimolar

mcM = micromolar

 

Afaik, the MW of DPPH is 394.33g

A 1 molar solution is a solution in which 1 mole of a compound is dissolved in a total volume of 1 litre.

A 1 molar solution DPPH will require 394.33g in 1 litre of solution

 

Approximately –

 

Weigh 0.394g of DPPH, dissolve  and make up to 1000ml with solvent = 1mMsolution DPPH = "A"

Pipette 1ml of A and make up to 1000ml with solvent = 1mcM solution = "B"

 

60ml of B will contain 60micromoles of DPPH


Kind Regards,

 

Charles.C


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