Hello everyone.
Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?
Thank you
Posted 05 June 2016 - 04:16 AM
Hello everyone.
Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?
Thank you
Posted 05 June 2016 - 07:17 AM
Hello everyone.
Can anyone share how to prepare 60 microM of DPPH in order to determine antioxidant activity?
Thank you
Hi rossy,
I assume you are familiar with diluting techniques.
I assume you are not requiring ultimate accuracies, otherwise maybe read this link –
http://www.pharmtech...-your-dilutions
http://chemistry.sta...olutions-method
M = molar
mM = millimolar
mcM = micromolar
Afaik, the MW of DPPH is 394.33g
A 1 molar solution is a solution in which 1 mole of a compound is dissolved in a total volume of 1 litre.
A 1 molar solution DPPH will require 394.33g in 1 litre of solution
Approximately –
Weigh 0.394g of DPPH, dissolve and make up to 1000ml with solvent = 1mMsolution DPPH = "A"
Pipette 1ml of A and make up to 1000ml with solvent = 1mcM solution = "B"
60ml of B will contain 60micromoles of DPPH
Kind Regards,
Charles.C
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