Hi everyone. I'm looking for standard operationg procedure for environmental swab test for e-coli, staphylococcus aureus and coliform.
thanks in advance
Posted 28 October 2017 - 06:25 AM
Hi everyone. I'm looking for standard operationg procedure for environmental swab test for e-coli, staphylococcus aureus and coliform.
thanks in advance
Posted 28 October 2017 - 06:51 AM
Hi
Hope this procedure may help you.
SOP FOR COLLECTION OF SWAB SAMPLE
Objective:
To lay down the procedure for collection of swabs sample from surface of equipment’s and floor
Scope:
This SOP shall be applicable to all the operational areas which are in contact with the product
Responsibility:
Microbiologist is responsible to implement this document.
Accountability:
Quality Assurance Manager.
Requirements:
ü Sterile swabs
ü Normal Saline or phosphate buffer solution
Procedure:
ü Take a sterile and sealed cotton swab,
ü Add 2-3 ml phosphate buffer saline having pH 7.2 or Normal saline (Sterilized),
ü Take the sterilized wet swab to the sampling area,
ü Wear hand gloves, take out the swab from the tube and swab the surface of the equipment/floor, covering an area of 10x10 sq.cm, in unidirectional movements but not to and fro movements,
ü After swabbing place the swab in tube containing normal saline or phosphate buffer saline
ü Shake the tube gently
ü Label the swab with appropriate sampling position and date.
ü Transfer the swab to the lab immediately,
ü Swab sample should be tested within 24 hours.
Acceptable limits:
After cleaning acceptable limits
TVC <100 cfu/cm2
E.coli <10 cfu/cm2
Staph <100 cu/cm2
Halal, ISO & Food Safety Consultant
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Posted 28 October 2017 - 07:38 AM
thank you. it would be good if u have sop for these specific microbs. Btw, thanks for replying. This help too.
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