4 replies to this topic

[Hasmukh Amin]

Hi,

 

Usually in a food sample Salmonella is tested per 25 gm ,However we have requirement to report it Salmonella /250 gm

kindly share method/protocol Or the changes we will have to make in the methods usually we follow BAM

 

Regards

Trupti

[jdpaul]

I believe it would be the enumeration of 10x 25gm samples and then reported as enumeration of 250 gm

Edited by jdpaul, 21 June 2018 - 01:19 PM.

[Charles.C]

Hi,

 

Usually in a food sample Salmonella is tested per 25 gm ,However we have requirement to report it Salmonella /250 gm

kindly share method/protocol Or the changes we will have to make in the methods usually we follow BAM

 

Regards

Trupti

 

Typo ??

[Charles.C]

addendum,

 

If 250gm is used and composited, probably need to adjust the volume of enrichment broth as detailed in BAM, eg -

 

1) The number and weights of the sample units is correct.

Each sample should be mixed to ensure homogeneity before withdrawing a 25 g analytical unit. The analytical units can be composited (fifteen 25 g units into a 375 g composite), unless otherwise indicated in Chapter 5 or in the OMA. Samples should be preenriched at a 1:9 sample-to-broth ratio.

 

(one common problem is lack of suitable size containers).

 

Alternatively, do individual x 10.

[Bo16]

We are also asked to run Salmonella in bulk quantities (375g is our usual request) as well.  For 250g, you can run 10 individual samples, or you can make the 1:10 dilution with 250 g sample to 2250ml of buffer.  This way you only have to transfer/streak the one sample.  We currently send the sample out to Covance Laboratories to run this bulk testing.  We only run it 8 or 10 times a year, and they only have a small up-charge to run the larger volume.  We have them run a method suitability once per product and then we have proof the method works.  Still cheaper and a better use of our Microbiologists time than running 10 (or in our case 15) separate Salmonella samples.