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Quantitative Environment Allergen Test

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cmo088

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Posted 17 October 2019 - 02:09 PM

I'm a high school student trying to craft my science fair research project. I want to perform an experiment to test different cleaning methods on the presence of the peanut allergen on a non-porous surface. I was doing some research, and all of the available environmental test kits appear to be qualitative or semi-quantitative. One of the project requirements is that the dependent variable must be quantitative. Is there any available environmental allergen test that is quantitative? (I have checked Neogen, maybe I missed something) The cheaper the better, however I understand that a test that meets these specifications will be somewhat expensive.



Charles.C

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Posted 17 October 2019 - 02:26 PM

I'm a high school student trying to craft my science fair research project. I want to perform an experiment to test different cleaning methods on the presence of the peanut allergen on a non-porous surface. I was doing some research, and all of the available environmental test kits appear to be qualitative or semi-quantitative. One of the project requirements is that the dependent variable must be quantitative. Is there any available environmental allergen test that is quantitative? (I have checked Neogen, maybe I missed something) The cheaper the better, however I understand that a test that meets these specifications will be somewhat expensive.

 

Hi cmo,

 

Yes, you maybe missed something, Or perhaps it depends on semantics.

 

From Neogen documentation -

 

B.   Quantitative tests

  Neogen’s Veratox and BioKits test kits determine the concentration of a target food allergen in ingredients, liquids, clean-in-place rinses and in finished foods.

1.  Veratox.  These  tests  are  microwell  ELISA  kits  that  detect  a  target  food  allergen  in  about  30 minutes following extraction. Following the test procedure, color changes in the sample wells are compared to the standards in the control wells using a microwell reader. Exact food allergen concentrations in the samples are computed using the comparisons.

2.  BioKits.  Microwell  ELISA  assay  test  kits  that  detect  target  allergens  in  approximately 1½ hours following extraction

 

 

Attached File  Neogen - Food_Allergen_Handbook.pdf   864.68KB   23 downloads


Kind Regards,

 

Charles.C


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The Food Scientist

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Posted 17 October 2019 - 06:38 PM

ELISA is a quantitative method. 

 

 

https://www.romerlab...rgen-test-kits/


Everything in food is science. The only subjective part is when you eat it. - Alton Brown.


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fepuhe

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Posted 18 October 2019 - 03:46 AM

Hope this could help

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