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Enrichment method for sulfite-reducing anaerobes from Lactic Acid Bacteria product?

Started by Elise11, Apr 24 2020 04:09 PM
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Elise11

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Posted 24 April 2020 - 04:09 PM

I have a food product that contais high amount of Lactic Acid Bacteria (LAB). I try to develop method for detecting sulfite-reducing anaerobes from that product but LAB prevents added C. perfringens growth. Any growth broth or conditions that allows sulfite-reducing anaerobes to grow but inhibits LAB to grow?


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mothershipwit

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Posted 30 April 2020 - 04:50 PM

If you enumerate directly from food to TSC agar per BAM chapter 16 you don’t get any C perf growth? Are you expecting C perf?


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Elise11

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Posted 06 May 2020 - 10:00 AM

If you enumerate directly from food to TSC agar per BAM chapter 16 you don’t get any C perf growth? Are you expecting C perf?

The problem is that customer needs result not detected/25g. With direct inoculation 1 g product we have gained around 50 % recovery for inoculated C. perfringens.


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