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Limits for Hand Hygiene Test by TSA contact plates


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maciejjaroszewski

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Posted 17 August 2020 - 02:40 AM

This week I'm planning to do the hand hygiene test and will be using TSA contact plates. We sample the surface of inner palm by formly pressing the agar against the three test area. I will count the colony. What guys you think about limit? What number is satisfy and what is not satisfy?

Thanks so much



Marloes

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Posted 17 August 2020 - 12:23 PM

Hi,

 

It also depends on when you are doing these hand hygiëne tests (after handwashing, during production) and if it is the latter it also depends on the microbiological load of your product.

We found the test during production unsuitable for cured products.

For our company we use the following chart:

  • 0-3 colonies 
    excellent
  • 4 -13 colonies
    good
  • 14-41 colonies
    moderate
  • 41-130
    insufficiënt
  • 131+
    bad

Good luck!



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olenazh

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Posted 17 August 2020 - 12:29 PM

Agree with Marloes: it's ineffective in cured/fermented product manufacturing environment. I swab employees' hands for E. Coli, limit less than 10.



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maciejjaroszewski

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Posted 18 August 2020 - 01:48 AM

Thank you for the reply and Im sorry I havent been more specific.

The test will be taken after handwashing. Most product is dairy (roux - mix of butter and flour).



Charles.C

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Posted 18 August 2020 - 02:09 PM

This week I'm planning to do the hand hygiene test and will be using TSA contact plates. We sample the surface of inner palm by formly pressing the agar against the three test area. I will count the colony. What guys you think about limit? What number is satisfy and what is not satisfy?

Thanks so much

 

hi macie,

 

see this thread and particularly Post 8 -

 

https://www.ifsqn.co...ces/#entry60958

 

(the specific process might also have some influence, eg RTE/NRTE)


Kind Regards,

 

Charles.C


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