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Acceptable level of Bacillus bacteria in the environment EMP

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suhaibalbadawi97

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Posted 04 September 2023 - 05:54 AM

Hi all..
Can you help me to determine the acceptable level of Bacillus bacteria in the environment EMP
thanks


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Charles.C

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Posted 04 September 2023 - 06:10 AM

Hi all..
Can you help me to determine the acceptable level of Bacillus bacteria in the environment EMP
thanks

Why do you envisage having significant Bacillus spp in your Environment ?

 

It is almost impossible to meaningfully respond to yr query without some knowledge of your Product/Process etc ?


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Kind Regards,

 

Charles.C


suhaibalbadawi97

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Posted 04 September 2023 - 06:44 AM

I'm sorry, I work in the catering and warehousing sector


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suhaibalbadawi97

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Posted 04 September 2023 - 07:27 AM

Why do you envisage having significant Bacillus spp in your Environment ?

It is almost impossible to meaningfully respond to yr query without some knowledge of your Product/Process etc ?


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Charles.C

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Posted 04 September 2023 - 12:07 PM

I'm sorry, I work in the catering and warehousing sector

No problem, IMEX Bacillus spp is not a particularly common environmental contaminant of interest within food processing facilities hence my query in Post 2. The most well-known (food pathogen) species in this genus is B.cereus.


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Kind Regards,

 

Charles.C


suhaibalbadawi97

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Posted 04 September 2023 - 03:50 PM

No problem, IMEX Bacillus spp is not a particularly common environmental contaminant of interest within food processing facilities hence my query in Post 2. The most well-known (food pathogen) species in this genus is B.cereus.


ok thanks but what is the limit for bacillus cereus ?

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Tony-C

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Posted 05 September 2023 - 03:18 AM

Hi suhaibalbadawi97,

 

I wouldn’t be getting hung up on Bacillus cereus, usually simply swabbing for indicators using a TVC and Enterobactericeae test are sufficient. I would also include Y&M’s if a product is susceptible to them.

 

Have a look at this guidance posted by Food Standards Scotland - Environmental Swabbing, I think you will find it useful.

 

“In a high proportion of cases a total count of aerobic mesophiles (TCAM, also commonly called a total viable count [TVC] or most simply a total count) is a suitable indicator. Using TCAM as an indicator is one of the cheapest tests offered by the testing lab, if a pour plate methodology is used.”

 

Other common indicators include Enterobacteriaceae - See Table 2 Recommendations and guidance for clean environmental surfaces. Criterium:

≤10 cfu/cm² for TCAM (TVC)

≤1 cfu/cm² for the Enterobactericeae

 

Kind regards,

 

Tony


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suhaibalbadawi97

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Posted 05 September 2023 - 06:05 AM

[quote name="Tony-C" post="198863" timestamp="1693883890"]

Hi suhaibalbadawi97,

I wouldn’t be getting hung up on Bacillus cereus, usually simply swabbing for indicators using a TVC and Enterobactericeae test are sufficient. I would also include Y&M’s if a product is susceptible to them.

Have a look at this guidance posted by Food Standards Scotland - Environmental Swabbing, I think you will find it useful.

“In a high proportion of cases a total count of aerobic mesophiles (TCAM, also commonly called a total viable count [TVC] or most simply a total count) is a suitable indicator. Using TCAM as an indicator is one of the cheapest tests offered by the testing lab, if a pour plate methodology is used.”

Other common indicators include Enterobacteriaceae - See Table 2 Recommendations and guidance for clean environmental surfaces. Criterium:
≤10 cfu/cm² for TCAM (TVC)
≤1 cfu/cm² for the Enterobactericeae

Kind regards,

Tony[/quote

thanks tony 👋]


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Charles.C

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Posted 05 September 2023 - 02:56 PM

[quote name="Tony-C" post="198863" timestamp="1693883890"]

Hi suhaibalbadawi97,

I wouldn’t be getting hung up on Bacillus cereus, usually simply swabbing for indicators using a TVC and Enterobactericeae test are sufficient. I would also include Y&M’s if a product is susceptible to them.

Have a look at this guidance posted by Food Standards Scotland - Environmental Swabbing, I think you will find it useful.

“In a high proportion of cases a total count of aerobic mesophiles (TCAM, also commonly called a total viable count [TVC] or most simply a total count) is a suitable indicator. Using TCAM as an indicator is one of the cheapest tests offered by the testing lab, if a pour plate methodology is used.”

Other common indicators include Enterobacteriaceae - See Table 2 Recommendations and guidance for clean environmental surfaces. Criterium:
≤10 cfu/cm² for TCAM (TVC)
≤1 cfu/cm² for the Enterobactericeae

Kind regards,

Tony[/quote

thanks tony ]

 

Of course, depending on precisely what is in the unknown environment ++, Pathogens could also be involved in the EMPG,


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Kind Regards,

 

Charles.C




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