5 replies to this topic

[AmyB]

I work for a juice manufacturer. We are currently researching millipore (filter) plating for our finished product, however we have not had any success with filtering pulpy product. Has anyone come across a methodology that would allow for millipore type plating of pulpy juice products?

Thank you,
Amy

[Charles.C]

Dear Amy,

Welcome to the forum !

Sorry that no responses so far. It is possible that yr query is within very narrow area. i did try googling the topic but the results were somewhat baffling in a technical sense (to me anyway).

Hopefully something may yet turn up.

Rgds / Charles.C

[Betty8]

 Hi Amy. I wish I could help you but in my company we using standard pour plate method for our finished product. We never had problems with micro results but we've tried millipore as well - unfortunately unsuccessful. Can I ask you what's the reason for using millipore ? Why you want to use that method?   


Betty

[Jean]

Dear Amy,


I too used to do pour & spread plating techniques for fruit juices. The reason being, 47mm thickness of the filter paper has very small pore size to filter pulpy juices. I used to dilute the fruit juice with sterile peptone water or phosphate buffer. For isolating bacteria pour plate technique and for fungal isolates spread plate is used.

[Minnie]

I work for a juice manufacturer. We are currently researching millipore (filter) plating for our finished product, however we have not had any success with filtering pulpy product. Has anyone come across a methodology that would allow for millipore type plating of pulpy juice products?

Thank you,
Amy

Hi Amy

In the past I have only ever been able to use a pour plate or spread plate method after diluting with sterile buffer solution. We tried the membranes but they just blocked up.

Regards

Minnie

[AmyB]

Hi Amy. I wish I could help you but in my company we using standard pour plate method for our finished product. We never had problems with micro results but we've tried millipore as well - unfortunately unsuccessful. Can I ask you what's the reason for using millipore ? Why you want to use that method?


Betty

Our finished product is pasteurized and therefore if there are any surviving organisms they would be few and far between. We would like to be able to filter plate a larger volume to ensure we are picking up any living organisms.