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Culture media for E. coli


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Gao Yu Qing

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Posted 18 May 2010 - 04:03 PM

I'm thinking of making culture slants for E.coli positive controls so I have a reproducing supply instead of wasting culti-loops all the time. What media/agars have you found that works best for you? Or do you keep your positive controls in a broth?


Edited by Gao Yu Qing, 18 May 2010 - 04:03 PM.


Charles.C

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Posted 21 May 2010 - 05:23 AM

Dear Gao Yu Qing,

You actually use positive controls ? I'm impressed :biggrin:

I just follow BAM, ICMCF or variants of.

Rgds / Charles.C


Kind Regards,

 

Charles.C


poppysnoss

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Posted 21 May 2010 - 04:50 PM

Hi.

I use Pro-cult discs and maintain them in nutrient broth, NA slopes and TBX agar for up to a month.

I use a fresh disc at the beginning of each month as our accreditation body won't let us (for obvious reasons) go more than one passage from NCTC or equivalent.

Pops



Gao Yu Qing

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Posted 26 May 2010 - 02:17 PM

For the curious, decided on NA as media of choice. Our method is to use a culti-loop to inoculate 3 slants. vial A is used the first week, vial B the second, and Vial C is used to inoculate the next 2 weeks of slants. We'll do this for 5 generations then start over.
I need to actually get ahold of a copy of the BAM and/or ICMCF. Think I'm going to hunt for online versions soon as I post this.






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