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Culture media for E. coli


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#1 Gao Yu Qing

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Posted 18 May 2010 - 04:03 PM

I'm thinking of making culture slants for E.coli positive controls so I have a reproducing supply instead of wasting culti-loops all the time. What media/agars have you found that works best for you? Or do you keep your positive controls in a broth?


Edited by Gao Yu Qing, 18 May 2010 - 04:03 PM.


#2 Charles.C

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Posted 21 May 2010 - 05:23 AM

Dear Gao Yu Qing,

You actually use positive controls ? I'm impressed :biggrin:

I just follow BAM, ICMCF or variants of.

Rgds / Charles.C


Kind Regards,

 

Charles.C


#3 poppysnoss

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Posted 21 May 2010 - 04:50 PM

Hi.

I use Pro-cult discs and maintain them in nutrient broth, NA slopes and TBX agar for up to a month.

I use a fresh disc at the beginning of each month as our accreditation body won't let us (for obvious reasons) go more than one passage from NCTC or equivalent.

Pops



#4 Gao Yu Qing

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Posted 26 May 2010 - 02:17 PM

For the curious, decided on NA as media of choice. Our method is to use a culti-loop to inoculate 3 slants. vial A is used the first week, vial B the second, and Vial C is used to inoculate the next 2 weeks of slants. We'll do this for 5 generations then start over.
I need to actually get ahold of a copy of the BAM and/or ICMCF. Think I'm going to hunt for online versions soon as I post this.






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