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Microbiological test procedure, best analysis procedure for Salmonella


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#1 hygienic

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Posted 05 February 2011 - 06:42 AM

Hi :

As every boday knowes ,Salmonella is a bacterium can cause an intestinal illness in people, we are doing lab tests to identify 3 types of bacteria , and I am looking for the best analysis procedure for Salmonells (the analysis method followed by us is Pour plating ) so can you tell what is the procedure includes the best and common Media using in this test ? our food includes raw poultry , raw meat , veal , cooked chicken , and cooked meat .


Thanks & Regards
Hygienic




#2 Charles.C

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Posted 05 February 2011 - 06:57 AM

Dear hygienic,

There is no unique answer to yr question.

One option which I'm sure you're lab. know well -

http://www.fda.gov/F...M/ucm070149.htm

Rgds / Charles.C


Kind Regards,

 

Charles.C


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#3 majoy

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Posted 08 February 2011 - 09:16 AM

Hi there!

For detection of salmonella, we use salmosyst broth for non-selective enrichment of the sample. A tablet of salmosyst-selective supplement is then added to the pre-enriched culture for selective enrichment. It is incubated in 35degC. For isolation, a loopful of the enriched broth is streaked to Rambach agar. Plates are incubated at 35degC for 18-48 hours. Pink to red colonies are observed as presumptive colonies. For confirmatory, use TSIA and LIA for suspected colonies.

Hope this helps.

Thanks thanks,


Hi :

As every boday knowes ,Salmonella is a bacterium can cause an intestinal illness in people, we are doing lab tests to identify 3 types of bacteria , and I am looking for the best analysis procedure for Salmonells (the analysis method followed by us is Pour plating ) so can you tell what is the procedure includes the best and common Media using in this test ? our food includes raw poultry , raw meat , veal , cooked chicken , and cooked meat .


Thanks & Regards
Hygienic




"Whatever you do, do it well..." - Walt Disney


#4 Charles.C

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Posted 08 February 2011 - 11:08 AM

Dear majoy,

Thks for input.

For confirmatory, use TSIA and LIA for suspected colonies.



I think full confirmation normally necessitates serological / additional biochemical testing.

Rgds / Charles.C

Kind Regards,

 

Charles.C


#5 poppysnoss

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Posted 08 February 2011 - 10:23 PM

Hi.

The testing I have used in the past for Salmonella spp, is as follows:

25g sample - 1:10 primary enrichment with Buffer Peptone Water, followed by subculturing to RVS and MKTTn broths. After incubation, broths plated onto Brilliant Green and XLD agar and any typical colonies are subject to confirmation, using agglutination & biochemical gallery.

Hope this helps. :smile:

Pops


Edited by poppysnoss, 10 February 2011 - 09:30 PM.


#6 sudarshan

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Posted 10 February 2011 - 11:43 AM

Dear Friend

PCR also good & rapid method for salmonella detection

http://www.ncbi.nlm....cles/PMC120641/

Sudarshan

Hi :

As every boday knowes ,Salmonella is a bacterium can cause an intestinal illness in people, we are doing lab tests to identify 3 types of bacteria , and I am looking for the best analysis procedure for Salmonells (the analysis method followed by us is Pour plating ) so can you tell what is the procedure includes the best and common Media using in this test ? our food includes raw poultry , raw meat , veal , cooked chicken , and cooked meat .


Thanks & Regards
Hygienic




Best Regards
Sudarshan Koli
koli.sudarshan@gmail.com

#7 Charles.C

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Posted 10 February 2011 - 12:10 PM

Dear All,

There seems to be a lot of rich laboratories reading this thread ! :biggrin:

Rgds / Charles.C


Kind Regards,

 

Charles.C


#8 hygienic

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Posted 11 February 2011 - 07:33 PM

Hi there!

For detection of salmonella, we use salmosyst broth for non-selective enrichment of the sample. A tablet of salmosyst-selective supplement is then added to the pre-enriched culture for selective enrichment. It is incubated in 35degC. For isolation, a loopful of the enriched broth is streaked to Rambach agar. Plates are incubated at 35degC for 18-48 hours. Pink to red colonies are observed as presumptive colonies. For confirmatory, use TSIA and LIA for suspected colonies.

Hope this helps.

Thanks thanks,





Really thanks alot , good lab tests knoweldge.

Regards

#9 majoy

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Posted 15 February 2011 - 09:55 AM

I agree Charles, PCR would be best for confirmatory...

But we are not a rich laboratory, so we do the LIA and TSIA :doh:


Majoy


Dear majoy,

Thks for input.



I think full confirmation normally necessitates serological / additional biochemical testing.

Rgds / Charles.C


"Whatever you do, do it well..." - Walt Disney





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