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Spiking food samples


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#1 Jennifer 1979

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Posted 31 July 2012 - 03:32 PM

Can anyone tell me thes best way to spike food samples for internal quality control monitoring.
I generally spike 10g food sample with 2ml of 10-6 dilution say E.coli but I am geting bad recovery. I always get good Z scores from EQA so dont understand why I get bad recoveries from spiking samples.



#2 Katja

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Posted 06 August 2012 - 03:42 PM

Can anyone tell me thes best way to spike food samples for internal quality control monitoring.
I generally spike 10g food sample with 2ml of 10-6 dilution say E.coli but I am geting bad recovery. I always get good Z scores from EQA so dont understand why I get bad recoveries from spiking samples.


are you sure your food actually supports the growth of the spiked micro-organism, maybe there is too much salt and too little water activity to support it. when did you test it, what is immediately after spiking or did you wait.




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