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Procedure and equipment for measuring Lobster Blood Protein


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#1 C.Arsenault

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Posted 30 April 2015 - 10:48 PM

HI

I am inquiring to see if anyone has any knowledge of information on procedures and equipment for measuring lobster blood protein.

 

Thanks

C :shades:



#2 MWidra

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Posted 01 May 2015 - 12:04 AM

If you are interested in the major crustacean allergen, this company makes a kit that will detect it from any source.  People allergic to shrimp are also allergic to lobster and crab.  So the kit will do it all.

 

You would need an ELISA plate reader to perform this, as well as pipettes and other laboratory equipment.  Their technical support people could probably help you.

 

http://www.elisas.com.au/ 

 

Here's another kit.

 

http://www.neogen.co...-Crustacea.pdf 

 

There are a lot of these kinds of kits out there, but most of them are ELISA kits, and use a plate reader.  If you have not ever done an ELISA before, I suggest that you talk to the manufacturer for some kind of training.

 

There are a lot of services out there that will test for these allergens as well.

 

You asked about lobster "blood".  Lobsters don't have blood, they have hemolymph, btw.  But the protein that people are allergic to in crustaceans is from the muscle, it appears.

 

You did not say why you needed it, so I'm not sure what you are looking for.

 

Martha 


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#3 xylough

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Posted 01 May 2015 - 01:28 AM

C Arsenault,

 

These two  papers seem to discuss lobster blood protein, not having to do with allergens, but rather as a measure of lobster quality.

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#4 MWidra

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Posted 01 May 2015 - 03:08 AM

C Arsenault,

 

These two  papers seem to discuss lobster blood protein, not having to do with allergens, but rather as a measure of lobster quality.

If this is what you are looking for, then you would need to know how to draw hemolymph from a lobster and read it on a hand-held refractometer that reads blood protein.  This refractometer mentions using it for lobster blood.

 

http://www.qasupplie...l#.VULs-NKrSCg 

 

To draw blood/hemolymph from a lobster, you will need to learn the technique, and syringes and needles.  I suspect that a marine biologist could tell you, or a company that uses this technique to assess the quality of their lobsters.

 

Martha


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