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Kindly share the procedure for doing open plate method


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#1 rani s r

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Posted 10 August 2015 - 03:46 PM

dear all,

kindly share the procedure for doing open plate method(passive airsampling).

 

thanks and regards

rani



#2 Charles.C

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Posted 10 August 2015 - 04:11 PM

dear all,

kindly share the procedure for doing open plate method(passive airsampling).

 

thanks and regards

rani

Hi rani,

 

Are you referring to micro. settle plates ?

 

can you provide some info. as to the specific application ?

 

It may depend on whether a specific micro. target or not but the general procedure is to use a standard nutrient agar and leave exposed for a fixed period. Then count.


Kind Regards,

 

Charles.C


#3 rani s r

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Posted 10 August 2015 - 05:05 PM

hai,

open plate method for air sampling for the identification of general bacterias present in air.i preform the method by open the petriplate containing nutrient agar for one hour and after that i incubate  the plates at 37 degree celsius for 48 hours. is this enough for air sampling.how can i report the reslt. is it enough to report the result in cfu/m3.



#4 Charles.C

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Posted 10 August 2015 - 05:17 PM

hai,

open plate method for air sampling for the identification of general bacterias present in air.i preform the method by open the petriplate containing nutrient agar for one hour and after that i incubate  the plates at 37 degree celsius for 48 hours. is this enough for air sampling.how can i report the reslt. is it enough to report the result in cfu/m3.

Hi rani,

 

strictly you need time units.

 

see this post - 

 

http://www.ifsqn.com...indpost&p=83052


Kind Regards,

 

Charles.C


#5 syju28380

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Posted 17 August 2015 - 08:50 AM

Hi Rani,

 

You may have a look at these standards

 

ISO 14698 Part 1-Clean rooms and associated controlled environments-Bio Contamination Control (I don't have link to a free download)

 

ISO 13408 Part 1-Aseptic processing of healthcare products- http://www.ctslab.eu...3408-1_2008.pdf(I am not sure whether the version is the latest)

 

Syju






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