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Liquid Formulation of Vibrio sp.

Liquid Formulation of Vibrio

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#1 green15b

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Posted 26 June 2016 - 02:57 AM

Dear All, 
I am trying to preverve Vibrio sp. in liquid formulation (in vegetative form). This is a salt tolerant vibrio and I am preserving the vibrio in 2% NaCl containing water. The preservation is done by diluting the culture broth (could not talk about exact composition - but it contain a mix of easy and difficult to ferment sugars) in the ratio of 1:5 in 2% NaCl water. 
This is a aerobic bacteria, but after preservation, due to closed environment there is now room for air exchange. 
I could see that the formulation keeps the viability of culture for 2 weeks but loosing the viability after that. 
 
Any suggestions about process improvement or suggestions about completely new process is most welcome. Thank you all for your kind help. 
 
Regards,
Kumar


#2 Charles.C

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Posted 26 June 2016 - 05:40 AM

 

Dear All, 
I am trying to preverve Vibrio sp. in liquid formulation (in vegetative form). This is a salt tolerant vibrio and I am preserving the vibrio in 2% NaCl containing water. The preservation is done by diluting the culture broth (could not talk about exact composition - but it contain a mix of easy and difficult to ferment sugars) in the ratio of 1:5 in 2% NaCl water. 
This is a aerobic bacteria, but after preservation, due to closed environment there is now room for air exchange. 
I could see that the formulation keeps the viability of culture for 2 weeks but loosing the viability after that. 
 
Any suggestions about process improvement or suggestions about completely new process is most welcome. Thank you all for your kind help. 
 
Regards,
Kumar

 

 

Hi Kumar,

 

There are potentially a lot of variables involved, eg storage temperature, specific species, preservation media, target storage duration, etc.


Kind Regards,

 

Charles.C





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