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#1 ati

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Posted 10 November 2016 - 03:35 AM

Dear all,

 

We are unfamiliar with the microbiological testing and need your advise on what is the correct sampling method for conducting either for shelf life test and thermophillic test.

For your information we are producing non-carbonated canned drinks by retorting the products.

 

So, for the incubation test for thermophillic, do we have to incubate the sample immediately after the production or can we wait for a few days before incubate the sample at 55 deg C for 5 days or both of this method can be use?

 

For the shelf life test, is it same as above, need to incubate the samples immediately or wait for few days? 

 

 

Thank you.

 

 



#2 Charles.C

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Posted 10 November 2016 - 02:54 PM

Dear all,

 

We are unfamiliar with the microbiological testing and need your advise on what is the correct sampling method for conducting either for shelf life test and thermophillic test.

For your information we are producing non-carbonated canned drinks by retorting the products.

 

So, for the incubation test for thermophillic, do we have to incubate the sample immediately after the production or can we wait for a few days before incubate the sample at 55 deg C for 5 days or both of this method can be use?

 

For the shelf life test, is it same as above, need to incubate the samples immediately or wait for few days? 

 

 

Thank you.

 

Hi ati,

 

A  little more info. might assist, eg -

 

canned/bottled ?

 

shelf stable, ie commercially sterile ? if not, storage temperature/shelf-life ?

 

Juices not my area but procedures for canned foods do not appear to be concerned with the specific timing of starting the incubation.

 

afaik, the incubation test is not relevant to shelf-life testing of canned foods eg -

 

Opinions  differ  concerning  the  value  of  incubating  and  testing  a  small number of cans immediately after processing, but there is  no  doubt  that such incubation tests are of no value in ensuring the safety of shelf-stable canned foods. It must also be borne in mind that such limited incubation tests  will  reveal  only  gross  underprocessing  and/or  extensive  post-processing  contamination.
(Microorganisms in Foods ICMSF Vol2)

 


Kind Regards,

 

Charles.C


#3 ati

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Posted 11 November 2016 - 01:19 AM

Dear Charles.C, 

 

Thank you for your comment and advice.

 

We are producing beverages in cans and in commercially sterile environment.

FYI, the product is herbal tea.

 

So, there is no specific method when to incubate the sample before conducting the microbiological test for thermophillic?

Is there any necessity to incubate the sample immediately after the production?

Does the result of the samples are still valid if we let the samples at room temperature for few days before incubation?



#4 Charles.C

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Posted 11 November 2016 - 04:16 AM

Dear Charles.C, 

 

Thank you for your comment and advice.

 

We are producing beverages in cans and in commercially sterile environment.

FYI, the product is herbal tea.

 

I assume this product is shelf-stable, ie commercially sterile, eg -

http://www.registrar...a_Drink?lang=en

 

So, there is no specific method when to incubate the sample before conducting the microbiological test for thermophillic?

not "afaik" from a micro. POV. although it is good practice to follow a standard procedure if one exists.

Is there any necessity to incubate the sample immediately after the production?

not "afaik" from a micro. POV. although it is good practice to follow a standard procedure if one exists.

Does the result of the samples are still valid if we let the samples at room temperature for few days before incubation?

Yes "afaik" although it is good practice to follow a standard procedure if one exists.

. (Note that 55degC is presumably >> room temperature.)

 

Personally, in the absence of any product-specific information, I suggest you ask the analytical lab. who will handle yr samples regarding scheduling. Here is a general procedure for incubation tests on canned goods.-

http://www.fda.gov/F...s/ucm109398.htm

 


Kind Regards,

 

Charles.C


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