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Microbiology test method of indoor air by exposed plates

exposed agar plates for air t

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#1 ahmed alabboud

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Posted 27 January 2017 - 11:53 AM

good day dears:

I  want to know how we can test the quality of indoor air by microbiology test . as i know we can use method test of exposed Agar petri dishes but  i want to help me for more information about that test method and what the References.

thank you



#2 BrummyJim

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Posted 27 January 2017 - 01:59 PM

From my experience processing these in a major micro lab in the UK, they are processed in the same way as any poured/streaked plates or the same agar. The plates should be exposed for 30 minutes for sampling and are then incubated at the required time/temperature (e.g. 72 hours@250 for Rose Bengal yeasts & moulds, 48 hours @370 for Plate Count Agar TVC)



#3 ahmed alabboud

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Posted 28 January 2017 - 04:51 AM

THANK YOU MR BrummyJim



#4 ahmed alabboud

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Posted 29 January 2017 - 11:11 AM

good day dears :

I ask if we exposed petri dish for 30 minutes and the result was 15 colonies  how we can calculate  cfu/m3  kindly mention to references .

 

thanks


Edited by Charles.C, 03 February 2017 - 07:21 AM.
combined, see post 7


#5 Charles.C

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Posted 30 January 2017 - 08:06 AM

good day dears :

I ask if we exposed petri dish for 30 minutes and the result was 15 colonies  how we can calculate  cfu/m3  kindly mention to references .

 

thanks

 

Hi ahmed,

 

Afaik, settle plate data is currently more often used to calculate cfu/area/time.

Impaction techniques are commonly used  if cfu/m3 values are required.

 

Nonetheless, a value for cfu/cm3 for yr settle plate data can be estimated via Omeliansky's formula as shown in this post 18 –

 

 http://www.ifsqn.com...ria/#entry95042

(also see Post6/method2, Post17)

 

A reference illustrating use of the Omeliansky formula  is attached.

Attached File  Sedimentation with the Omeliansky formula,2012.pdf   44.61KB   52 downloads


Edited by Charles.C, 03 February 2017 - 07:21 AM.
combined, see post 7

Kind Regards,

 

Charles.C


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#6 ahmed alabboud

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Posted 31 January 2017 - 10:48 AM

thanks dear Charles.. best regards


Edited by Charles.C, 03 February 2017 - 07:21 AM.
combined, see post 7


#7 CMHeywood

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Posted 01 February 2017 - 10:00 PM

Also see this recent post: 

Indoor air test by exposed petri dishes

Started by ahmed alabboud, Jan 29 2017 05:11 AM
 
Sorry, I don't know how to give you a link to this post, but was in the recent email from IFSQN.

Edited by Charles.C, 03 February 2017 - 07:18 AM.
thanks, I have now combined the 2 threads





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