10 replies to this topic

[EagleEye]

Could someone please help me on this Yeast and Mold count trick?
Suggest me some sources where I can find some information regarding the Mold count..

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[Packaging QA]

Report as 'SPR' = spreader. Repeat the test , check plate at 48h , 72h and put a spot on the centre of juvenile colonies (dont open lid of plate). To get like that would usually take 5 plus days , but colonies would be smaller , visible and able to be counted much earlier.

[dfreund]

If this is obviously outside of tolerance limits, I would mark it TNTC (Too Numerous To Count) and mark it a sample that failed.

[EagleEye]

If this is obviously outside of tolerance limits, I would mark it TNTC (Too Numerous To Count) and mark it a sample that failed.

Thank you for the reply, dfreund..
This is spices mix sample's 10^3 dilution plate. All the lower dilutions are like this. Would you suggest to plate out higher dilutions so that would enable to get some colony distinctly?
Is there any supporting document regarding a detailed how-to-do method for Yeast and Mold count. I searched many standards but couldn't find one..

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[EagleEye]

Report as 'SPR' = spreader. Repeat the test , check plate at 48h , 72h and put a spot on the centre of juvenile colonies (dont open lid of plate). To get like that would usually take 5 plus days , but colonies would be smaller , visible and able to be counted much earlier.

Thank you "Packaging QA" (that's a crazy name!!!)

This is a 10^3 dilution 72 hour plate of a spices mix product. Why it is like this in your opinion? Is it because the species is fast growing or is denote the higher number present? Would a higher dilution help?
It seems to me as A.niger growth. I expect your expert opinion on this..
Thanks in advance,

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[dfreund]

We are not doing this in house but I expect this is good guidance:

 

https://www.fda.gov/...s/ucm071435.htm

[EagleEye]

We are not doing this in house but I expect this is good guidance:

https://www.fda.gov/...s/ucm071435.htm

Thank you dfreund, for the input.
I had seen this FDA manual but the detail about my confusion is specifically not answered there. The overall growth on the surface. Would you say it might be due to the higher number present in the sample, possibly? Or this might be the nature of the Mold?


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[Charles.C]

Hi Navas,

 

Y&M not my direct experience but various approaches to yr OP appear possible, eg -

 

(1) Use Petrifilm for which counting advice is supplied (ymc1,2 below) -

(2) inc dilution, eg ymc3

 

For some theory on handling "upper limits" can try ymc4.

 

 ymc1 Petrifilm Y&M Guide(1).pdf   324.57KB   6 downloads

 ymc2 - Petrifilm Y&M Guide(2).pdf   319.52KB   4 downloads

 ymc3 - Micro.examination -total colony number (Petrifilm).pdf   159.16KB   7 downloads

 ymc4 - accuracy plate counts.pdf   3MB   7 downloads

 

 

 

 

 

 

 

 

[EagleEye]

Hi Navas,

Y&M not my direct experience but various approaches to yr OP appear possible, eg -

(1) Use Petrifilm for which counting advice is supplied (ymc1,2 below) -
(2) inc dilution, eg ymc3

For some theory on handling "upper limits" can try ymc4.

ymc1 Petrifilm Y&M Guide(1).pdf
ymc2 - Petrifilm Y&M Guide(2).pdf
ymc3 - Micro.examination -total colony number (Petrifilm).pdf
ymc4 - accuracy plate counts.pdf

I really appreciates your help, Charles.
Now I am getting more focused to what answer I search for. I think, these documents would help to convince my Superior. I had that Petrifilm one, but others also feels really helpful..
Thank you again..

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[Packaging QA]

Thank you for the reply, dfreund..
This is spices mix sample's 10^3 dilution plate. All the lower dilutions are like this. Would you suggest to plate out higher dilutions so that would enable to get some colony distinctly?
Is there any supporting document regarding a detailed how-to-do method for Yeast and Mold count. I searched many standards but couldn't find one..

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You have a mold that is aggressively growing on the surface of the plate. The fact your lower dilutions look like this also doesnt necessarily mean there are high numbers. As I said, ask that the lab to count at 48/72H to spot the juvenile colonies before they get to the point where they are mature and spread over one another.

[EagleEye]

You have a mold that is aggressively growing on the surface of the plate. The fact your lower dilutions look like this also doesnt necessarily mean there are high numbers. As I said, ask that the lab to count at 48/72H to spot the juvenile colonies before they get to the point where they are mature and spread over one another.

Yes, I got the point now..

Thank you my friend....

Regards

-Navas


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