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Tests for Allergen Proteins in Celery

Celery Allergen

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#1 Bhuvanya B

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Posted 02 August 2018 - 05:14 AM

Are there any commercial methods available to test for the allergen proteins in Celery such as Prolifins and PR-10? Are any laboratories in Europe offering such a test?



#2 pHruit

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Posted 02 August 2018 - 08:49 AM

I know that one of our suppliers that processes celery uses Imegen (https://www.imegen.es/en/) for testing for their cleaning validation so could be worth contacting them.
Not too sure on exact method though, as we don't take anything from that site so never had to review in much detail.



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#3 Charles.C

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Posted 02 August 2018 - 09:57 AM

also see -

 

http://www.genesig.c...celery-celeriac

 

https://www.bc-diagn...-detection-kit/

 

https://food.r-bioph...lergens/celery/

 

http://www.ifsqn.com...-allergen-test/

 

http://www.ifsqn.com...elery-and-milk/

 

PCR can be used for allergens like celery which cannot be detected by antibodies. Celery has to be labeled in the EU but until now, all attempts to produce reliable antibodies have failed due to the close relationship between celery and other plants like parsley, carrot, coriander or fennel.
Over the last decade, newer DNA detection techniques have been developed. All these so-called isothermal amplification methods are in some way related to the conventional PCR but can be performed almost without any instrumentation.
A simple heating block is used to amplify the target DNA and the subsequent visual detection is realized via fluorescent dyes. Isothermal amplification is usually faster than PCR and less prone to any co-isolated impurities and in many cases even more sensitive.

 

https://www.romerlab...lergen-testing/


Kind Regards,

 

Charles.C


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#4 Bhuvanya B

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Posted 06 August 2018 - 05:42 AM

 

Thank you Charles, for all the details shared. But we a trying to establish if the celery allergen proteins such as PR-10 and Prolifins are present in a Parsley-Celery hybrid seed. We have already tested the seeds with PCR and Next Generation DNA Sequencing have positively detected the presence of celery DNA. But we also want to know if the celery allergen proteins are also present in the seeds and hence would like to know if there is any test method available wherein the celery protein can be detected. Basically we are trying to understand if such Parsley-Celery hybrid plants are safe for consumption by individuals who are allergic towards celery.


Edited by Bhuvanya B, 06 August 2018 - 05:44 AM.


#5 Charles.C

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Posted 06 August 2018 - 08:22 AM

Hi Bhunvanya B.

 

Yr query sounds like an excellent teaser to pass on to Romer lab. (If they exist in India).


Kind Regards,

 

Charles.C


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#6 Zahid Sarfraz1

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Posted 06 August 2018 - 08:27 AM

I think IDAC will be the better option for you...



#7 Charles.C

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Posted 06 August 2018 - 08:31 AM

I think IDAC will be the better option for you...

 

Do you mean -

 

http://www.idac.org/ 


Kind Regards,

 

Charles.C


#8 Charles.C

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Posted 06 August 2018 - 08:46 AM

Hi Zahid,

 

Please supply link in Romanic language.


Kind Regards,

 

Charles.C






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