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Growth Promotion Test of media for microbiological Analysis


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#1 BALA Prasanna

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Posted 29 June 2019 - 09:32 AM

Dear Team,

                   Currently we are using ready made powdered media for microbiological Analysis in Food industry.

 

Is it required to check the growth promotion test for those media?

 

If it is mandatory, how to maintain the culture in food industry? 

 

Anyone to clarify this.


Edited by KASI4bala, 29 June 2019 - 09:36 AM.


#2 Charles.C

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Posted 29 June 2019 - 03:34 PM

Check it for Whom ?

 

In Principle, ideally one should of course. But in practice ........it depends.......


Kind Regards,

 

Charles.C


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#3 LesleySR

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Posted 01 July 2019 - 09:39 AM

Hi there - you say you are using "ready made powdered media" - I am assuming that you mean that you buy the powdered media & combine with water to make the agar you require?.....

 

If you are purchasing ready poured plates you could rely on your supplier for a COA (showing that the microorganism of interest will grow on the plates) if you are making your own agar & are compliant with ISO17025 it is required that you check the efficacy of the media with a positive control.

 

https://www.iso.org/...dard/66912.html

 

I don't have a copy of this standard - you would need to purchase this or ask a laboratory that is accredited if they can give you a copy.

 

To obtain a positive control - these are available from specialist suppliers but as part of ISO17025 you need to keep these standards under your control & locked away - they can be expensive too.

https://www.sigmaald...organism-mk.pdf

 

 

If you are not ISO17025  accredited please be aware that may customers are now mandating that any test that is critical to food safety or legality is conducted in an ISO17025 accredited laboratory & BRC Version 8 now includes this too (9.3.4) so this may be something you may need to consider?....

 

 

 

 

Dear Team,

                   Currently we are using ready made powdered media for microbiological Analysis in Food industry.

 

Is it required to check the growth promotion test for those media?

 

If it is mandatory, how to maintain the culture in food industry? 

 

Anyone to clarify this.



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#4 Charles.C

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Posted 01 July 2019 - 12:08 PM

Hi there - you say you are using "ready made powdered media" - I am assuming that you mean that you buy the powdered media & combine with water to make the agar you require?.....

 

If you are purchasing ready poured plates you could rely on your supplier for a COA (showing that the microorganism of interest will grow on the plates) if you are making your own agar & are compliant with ISO17025 it is required that you check the efficacy of the media with a positive control.

 

https://www.iso.org/...dard/66912.html

 

I don't have a copy of this standard - you would need to purchase this or ask a laboratory that is accredited if they can give you a copy.

 

To obtain a positive control - these are available from specialist suppliers but as part of ISO17025 you need to keep these standards under your control & locked away - they can be expensive too.

https://www.sigmaald...organism-mk.pdf

 

 

If you are not ISO17025  accredited please be aware that may customers are now mandating that any test that is critical to food safety or legality is conducted in an ISO17025 accredited laboratory & BRC Version 8 now includes this too (9.3.4) so this may be something you may need to consider?....

 

Hi Lesley,

 

^^ Sorry to nitpick but this is not exactly correct.
 

 

Where the company undertakes or subcontracts analyses which are critical to product safety or legality,
the laboratory or subcontractors shall have gained recognised laboratory accreditation or operate in
accordance with the requirements and principles of ISO 17025
. Documented justification shall be
available where non-accredited test methods are used.

 

 

I'm uncertain exactly what BRC do mean by "recognised laboatory accreditation" or "documented justification". Maybe they require a proficiency (aka ring) test data.

 

I daresay the I.G. clarifies this.

 

As you say, positive controls come with "liabilities"

 

I also note that some externally reported micro. results based on kit-derived samples appear to not always be based on fully confirmed data.


Kind Regards,

 

Charles.C


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#5 LesleySR

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Posted 01 July 2019 - 01:21 PM

Charles 

 

I take your point on this & I'm not sure that a standard BRC auditor would actually understand what would constitute "documented justification" anyway - none of the BRC auditors I have met appear to be microbiology specialists.

 

Certainly in a laboratory setting (where you are ISO17025 accredited) to add a new method to the scope of your laboratory accreditation you have to prove that the new method will deliver the correct results against a standard ISO/AOAC when used for your product matrix....usually this means comparative testing & for pathogens might involve several different serotypes, this is a considerable amount of testing work to demonstrate compliance (especially if none of the tests offered by a laboratory are accredited)

 

The interpretation notes for BRC 8 offer this information.

 

Interpretation Analyses critical to safety and legality

 

 

This clause applies to tests which are critical to product safety or legality. Results from such tests must be credible and may be called upon in a court of law.

The company needs to identify which tests are critical to product safety or legality, such as compliance with label claims/declarations (e.g. nutritional claims, alcohol content) and tests for contamination (e.g. by pesticides, aflatoxins).

 

Note that while the laboratory itself may have accreditation, the actual test methods must also be accredited. Any method of analysis that is not accredited needs justification as to why it was used (e.g. it may be a method for which no accreditation is yet available). Where critical tests are carried out by non-accredited laboratories (either contracted or on-site laboratories), there must be suitable documentary assurances that the laboratory is working to the requirements and principles of ISO 17025.

 

This must include confirmation of the laboratory’s procedures to meet the following general principles:

 

 

• staff competency and documented training

• documented test methodology based on accepted standards

• equipment that is fit for purpose and appropriately calibrated

• a documented quality assurance programme, including paired testing, ring testing etc.

• completion of internal audits of the laboratory’s operation.

 

Thus, I think what BRC is trying to say is that your laboratory (and methods) may not be accredited but the laboratory itself has to work to ISO17025 standards & you would need documentation to prove this.

 

But as KASI4bala is based in India BRC might not be an issue & their customers might not have this requirement?

 

 

 

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#6 Charles.C

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Posted 01 July 2019 - 01:43 PM

Hi Lesley,

 

Thks yr info.

 

Yes, I agree with yr penultimate paragraph.

 

I anticipate it is sufficient that - 

 

(a) relevant micro. procedures are, for example, AOAC supportable.

(b) relevant lab personnel have documented training capabilities based on iso17025 standard,

(c) appropriate ring testing has been carried out

(d) lab function is included in internal audit.

 

And Yes, there was no reply to Post 2.


Kind Regards,

 

Charles.C


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#7 BALA Prasanna

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Posted 01 July 2019 - 03:11 PM

Hai thanks for your valuable reply.

 

Im am working as a Microbiologist in Food Industry. 

 

In the same premises we are tested the microbial parameters & manufactured foods.

 

If I am maintaining positive culture for this test, it may possible to contaminate my foods. If any outbreaks happen it may leads to thread in my foods.

 

This is inhouse lab for the industry. can you clarify more detail?



#8 LesleySR

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Posted 01 July 2019 - 03:50 PM

The standards for managing positive controls are held within ISO17025.

I would advise you to try to obtain a copy of this document as it is very useful to know what controls are needed to minimize contamination of the factory & of other samples.

Every accredited laboratory has to have these positive controls & with care there shouldn't be any contamination. In the same we store cooked & ready to eat products above raw meats in our refrigerator, the same logic is used here.

This is also why the standards are kept in a locked fridge & when they are used to check agar efficacy they need to be carefully handled by trained staff, away from other samples & the work area cleaned thoroughly after use.



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#9 Charles.C

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Posted 01 July 2019 - 04:24 PM

Hi Lesley,

 

Unfortunately, IMEX, in some cases/locations, suitable positive/negative controls are not always readily available, eg V.parahaemolyticus.

 

in other cases, the "liability" can be  rather "sensitive", eg V.cholerae


Kind Regards,

 

Charles.C


#10 BALA Prasanna

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Posted 08 November 2019 - 06:16 AM

Dear Team,

                    My lab is ISO 17025 accrediated only in Chemical Parameter not Microbiological Parameter. 

 

Growth Promotion test we are not tested for the media.

 

We are using the media based on their COA.

 

Following data are the reason for not doing th GPT,

 

1. Verifying Supplier COA

 

2. Approved Supplier (Ex: Himedia)

 

3. Inter lab Comparision in accrediated laboratories is matching with Inhouse laboratory. 

 

So that Our managment has conclude no requirment to check the GPT.

 

Is the justification ok - Any one pls suggest.

 


Edited by BALA Prasanna, 08 November 2019 - 06:17 AM.


#11 Charles.C

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Posted 09 November 2019 - 10:40 AM

also see -

 

https://www.ifsqn.co...ts/#entry151088


Kind Regards,

 

Charles.C





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