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Interpreting environmental swab E. coli results (ECC cfu/cm²): what’s the pass/fail limit?

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kconf

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Posted 21 January 2026 - 03:10 PM

Mind you this is exclusively for carcass. All the other regular Petri films are read as they appear, no further calculations. 


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GMO

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Posted 21 January 2026 - 04:25 PM

Ah ok, but still, explain for me, is it per cm2 of the carcass area swabbed or per cm2 of the petrifilm?

Why does it make a difference using petrifilm?


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kconf

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Posted 21 January 2026 - 04:56 PM

Area swabbed.

 

What do you mean why it makes a difference? Why does it get multiplied by 0.03? It has to do with dilution factor and carcass rinse. 


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GMO

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Posted 21 January 2026 - 06:55 PM

Area swabbed.

 

What do you mean why it makes a difference? Why does it get multiplied by 0.03? It has to do with dilution factor and carcass rinse. 

 

So it's from a rinse not a swab? But it says swab? Still not getting it. But I've been off topic enough!


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Posted 21 January 2026 - 08:33 PM

chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.fsis.usd...Swine_Estab.pdf

 

The best-sourced document is above for reference; The UCL/LCL should be based on historical trended data results, with your limits set accordingly.  


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GMO

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Posted 22 January 2026 - 06:32 AM

chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.fsis.usd...Swine_Estab.pdf

 

The best-sourced document is above for reference; The UCL/LCL should be based on historical trended data results, with your limits set accordingly.  

 

Thank you. That now makes sense.


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kconf

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Posted 22 January 2026 - 01:59 PM

That was one of my initial responses. Scampi shared it too. 


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Tony-C

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Posted 23 January 2026 - 05:27 AM

That was one of my initial responses. Scampi shared it too. 

 

:welcome: 

 

Welcome to my world, take it as a compliment   ;)

 

Kind regards,

 

Tony


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GMO

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Posted 23 January 2026 - 05:51 AM

:welcome:

 

Welcome to my world, take it as a compliment   ;)

 

Kind regards,

 

Tony

 

What is with the tone of people's responses to me recently on here? What is it I've done wrong Tony and others that you forget there is a real person? It's sad.


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GMO

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Posted 23 January 2026 - 05:59 AM

That was one of my initial responses. Scampi shared it too. 

 

I wrote two replies yesterday but deleted them both. I was confused and asking questions. I finally had time to look at the link and it made some sense albeit not with the responses previous people added which still don't make sense to me.

 

But to be honest, I was quite hurt. So what if I didn't have time to look at a link before? Does that matter? I'd kind of hope we'd like people who ask questions and try to understand as Technical people. But yesterday and today feels like I've obviously p---ed off you and others on here for some reason. If I have it's unintentional. 

 

Wow.


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Tony-C

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Posted 23 January 2026 - 06:25 AM

What is with the tone of people's responses to me recently on here? What is it I've done wrong Tony and others that you forget there is a real person? It's sad.

 

The comment wasn't directed at you GMO, it was a general comment.

 

Apologies if you thought it was directed at you and took it personally.

 

Kind regards,

 

Tony


Edited by Tony-C, 23 January 2026 - 06:33 AM.

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GMO

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Posted 23 January 2026 - 07:18 AM

"Apologies if you thought..."

 

Never a good start Tony. 

 

Whatever.


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kconf

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Posted 23 January 2026 - 02:12 PM

Relax, my friend. You didn't do anything to p--- me off. I was only saying that the info that made sense to you was no different than the info I had shared. And yes, we do encourage people to ask questions and keep asking until they understand. 


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Tony-C

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Posted Today, 06:47 AM

"Apologies if you thought..."

 

Never a good start Tony. 

 

Whatever.

 

:uhm:  My post had nothing whatsoever to do with you GMO.

 

Scampi posted a very good link and guideline:

 

Helpful to know where in the world you are but below should help

 

Table 3. Values for Marginal and Unacceptable Results for E. coli performance criteria Slaughter Acceptable Range Marginal Range Unacceptable Range Class Cattle *negative positive above 100 but not above 100 cfu/cm2 cfu/cm2 Swine 10 cfu/cm2 above 10 cfu/cm2 above 10,000 but not above 10,000 cfu/cm2 cfu/cm2 * An excised sample is considered negative when no E. coli colonies are present on plate(s) Of the lowest dilution (10- 1 1). If E. coli colonies are present, multiply the average plate count by the appropriate dilution factor and record the result as cfu/cm2 .

https://www.fsis.usd...Swine_Estab.pdf

 

Which was reposted here:

 

 

And here:

 

chrome-extension://efaidnbmnnnibpcajpcglclefindmkaj/https://www.fsis.usd...Swine_Estab.pdf

 

The best-sourced document is above for reference; The UCL/LCL should be based on historical trended data results, with your limits set accordingly.  

 

My comment 'Welcome to my world' was directed to konf as the links, information etc. that I have posted over the years tend to get copied and posted (rather than linking to the original). I have even pulled up people who have posted my documents.

 

I apologised if my post caused you any offence and you brushed that off which I do find unbecoming.

 

Kind regards,

 

Tony


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