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Detection of Salmonella for Dietary Supplements

rapid detection salmonella herbal supplements dietary supplements

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#1 rsatanowski

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Posted 08 June 2016 - 03:07 PM

Good morning,

 

I am being tasked with setting up a microbiology lab and establishing methods with virtually no budget and no knowledge of working with BSL2 bacteria. My company is aware I am not the best person for this job, so I thought I would reach out and see if anybody has advice.

 

My manager would like me to use 3M petrifilm instead of making my own media. They manufacture a rapid salmonella detection "kit" which is essentially pre-made media and they also provide a "confirmation disk" has anybody worked with this before? 

 

Additionally, am I able to safely create a positive control for this plate if we are a BSL 1 level lab? I would be purchasing lyophilized pellets. Is it necessary to make a positive control if the plates are validated by 3M? We are using the USP as our guideline and I am unsure if I need to conduct challenge testing for Salmonella. I believe I do. 

 

Any and all advice would be appreciated. 


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#2 Charles.C

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Posted 08 June 2016 - 06:13 PM

Hi rsatanowski,

I'm not in USA but based on previous threads here, running a micro.lab in a manufacturing facility in this location can be a very debatable enterprise. Especially for pathogenic species.

May I ask what kind of dietary products are involved ?

Regarding 3M, one relevant query might be if their product is AOAC accredited for Salmonella


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Charles.C


#3 Charles.C

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Posted 08 June 2016 - 06:44 PM

addendum - I presume you have some microbiological credentials ?

 

Regarding positive control, I think it is usually recommended to obtain a control which utlilises a relatively non-potent variety.

 

(I'm not familiar with BSL risk ratings, my knowledge in this respect is limited to a 1985 textbook which although highly respected is likely to  be out of date. At that time there was no globally harmonised system but other than 2 serotypes, Salmonella was [book] classified as level 2 which enabled investigation in a "basic" laboratory, ie non-containment. Nonetheless IMEX some US Companies have a Policy to simply ban microbiological labs from a food facility).


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Charles.C


#4 rsatanowski

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Posted 08 June 2016 - 07:05 PM

I do have experience in Microbiology, both formal and career related, however I have only worked in companies which had been well established. My thought too was that there must be laboratory "safe" strains of Salmonella for testing, however when searching online for such lyophilized pellets, they all have a safety rating for BSL 2. I believe I am able to order them without issue, but I am just trying to discern whether this is practical or safe as I have not worked with bacteria at that level. The condition set by the USP guideline is that I need to prove the absence of E.Coli and Salmonella. Our raw materials are botannicals in powder form. Such as Aloe Vera, Grapeseed, Tumeric. 


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#5 rsatanowski

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Posted 08 June 2016 - 07:07 PM

Oh, and I had not thought to check but 3M methods are AOAC approved. So thank you for that!


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#6 Charles.C

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Posted 08 June 2016 - 08:11 PM

Oh, and I had not thought to check but 3M methods are AOAC approved. So thank you for that!

 

Hopefully for your Product  variety? (usually the relevant AOAC procedure will specify what range of products are validated. Or known exceptions.)

 

"Absence" is a rather vague term. I daresay the USP has an associated sampling plan also.

 

From memory, S.typhimurium is a common food control, Difco et al are probably a good reference/source (for safety aspects also).

IMEX testing for Salmonella is not simple. One common problem with rapid kits is false positives.

 

The practicality/choice of methodology rather depends on the volume of samples anticipated IMO.


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Charles.C






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