I am being tasked with setting up a microbiology lab and establishing methods with virtually no budget and no knowledge of working with BSL2 bacteria. My company is aware I am not the best person for this job, so I thought I would reach out and see if anybody has advice.
My manager would like me to use 3M petrifilm instead of making my own media. They manufacture a rapid salmonella detection "kit" which is essentially pre-made media and they also provide a "confirmation disk" has anybody worked with this before?
Additionally, am I able to safely create a positive control for this plate if we are a BSL 1 level lab? I would be purchasing lyophilized pellets. Is it necessary to make a positive control if the plates are validated by 3M? We are using the USP as our guideline and I am unsure if I need to conduct challenge testing for Salmonella. I believe I do.
Any and all advice would be appreciated.