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Help interpreting e. coli reading results

e. coli almonds

Best Answer MsMars, 27 March 2018 - 04:57 PM

MPN - most probable number.  <0.3 MPN usually means that results are below detectable levels for that particular test. So can't say for sure that sample did not contain E. coli  at all (can't really ever say that), but that the levels were below detectable levels. 

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#1 svg87

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Posted 27 March 2018 - 04:40 PM

I have a question, a costumer requested an E.coli test on pasteurized almonds, this is the result from the lab:

E. Coli / AOAC 966.24

E. Coli 1 <0.3 /g MPN (what this means?)

 

so the 0.3 means that is contaminated with e. coli?



#2 MsMars

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Posted 27 March 2018 - 04:57 PM   Best Answer

MPN - most probable number.  <0.3 MPN usually means that results are below detectable levels for that particular test. So can't say for sure that sample did not contain E. coli  at all (can't really ever say that), but that the levels were below detectable levels. 



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#3 svg87

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Posted 27 March 2018 - 05:10 PM

thank you so much



#4 Charles.C

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Posted 28 March 2018 - 05:53 AM

I have a question, a costumer requested an E.coli test on pasteurized almonds, this is the result from the lab:

E. Coli / AOAC 966.24

E. Coli 1 <0.3 /g MPN (what this means?)

 

so the 0.3 means that is contaminated with e. coli?

 

Hi svg87,

 

Previous post2 is "operationally" correct.

 

Statistically the "<" means that the sets of tubes used to test for the species were all negative, ie no detection. Mathematically this does not yield a specific answer for the MPN.

 

So the (most likely) answer for the MPN is then "interpreted" as being less than the (most likely) value would have been if One tube had been positive. And that value in yr case was (apparently) 0.3cfu/gram.

 

PS - Just for illustration here is one set of tubes (3x3x3) where the (null) result was < 3 cfu/gram

 

Attached File  MPN table.png   83.68KB   0 downloads


Kind Regards,

 

Charles.C


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#5 svg87

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Posted 28 March 2018 - 01:16 PM

thanks Charles :spoton: 







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